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NIR-driven multifunctional PEC biosensor based on aptamer-modified PDA/MnO2 photoelectrode for bacterial detection
Anni Cui1, Lihua Dong2, Yiting Hou3
1Centre for Advanced Optoelectronic Functional Materials Research, Key Laboratory for UV Light-Emitting Materials and Technology of the Ministry of Education, Northeast Normal University, Changchun, 130024, China.
Biosensors & Bioelectronics
|April 25, 2024
Summary
A novel photoelectrochemical biosensor detects and inactivates Staphylococcus aureus (S. aureus) using a PDA/MnO2 photoelectrode. This system offers sensitive bacterial detection and achieves 97.36% inactivation via combined photothermal and catalytic effects.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biotechnology
Background:
- Foodborne bacterial infections, particularly from Staphylococcus aureus (S. aureus), pose a significant public health risk.
- Sensitive detection and effective bacterial inactivation are crucial for preventing foodborne illnesses.
Purpose of the Study:
- To develop a multifunctional photoelectrochemical (PEC) biosensor for the simultaneous detection and inactivation of S. aureus.
- To utilize a novel PDA/MnO2 photoelectrode functionalized with SA31 aptamers for enhanced performance.
Main Methods:
- Immobilization of carboxyl-functionalized SA31 aptamer onto a PDA/MnO2 photoelectrode via covalent bonding.
- Detection of S. aureus based on the steric hindrance effect leading to a photocurrent-off signal.
- Inactivation of S. aureus using the peroxidase-like activity of PDA/MnO2 with H2O2 and NIR-induced photothermal effects.
Main Results:
- The PEC biosensor achieved sensitive detection of S. aureus with a linear range from 10 to 10^7 CFU/mL and a low detection limit of 2.0 CFU/mL.
- The biosensor demonstrated effective inactivation of S. aureus, reaching 97.36% efficacy through a synergistic antibacterial mechanism.
- A 'signal on' switch was observed upon inactivation and desorption of S. aureus, indicating successful bacterial removal.
Conclusions:
- The developed NIR-driven PEC biosensor offers a promising strategy for sensitive detection and effective inactivation of S. aureus.
- The combination of aptamer recognition, peroxidase-like catalysis, and photothermal therapy provides a multifunctional platform for bacterial control.
- This approach holds potential for practical applications in food safety and public health to combat bacterial infections.
