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SARS-CoV-2 live virus culture and sample freeze-thaw stability
Phyllis J Kanki1, Donald J Hamel1, Stefan Riedel2
1Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 651 Huntington Avenue, Boston, MA 02115, USA.
Diagnostic Microbiology and Infectious Disease
|April 25, 2024
Abstract:
The effect of freeze-thaw on SARS-CoV-2 viral viability is not well established. We isolated virus from 31 split clinical samples cultured fresh or after a 7- or 17/18-day freeze. We found that freeze-thaw did not significantly affect viral culture isolation. Therefore, frozen samples may be used to assess SARS-CoV-2 infectiousness.

