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Identification of key mRNAs and signaling pathways in obsessive compulsive disorder based on weighted gene
Xiaoming Zhang1, Yanru Liu1, Bin Guo1
1Department of Psychiatry, Beijing Huilongguan Hospital, Beijing, China.
Purpose:
Obsessive-compulsive disorder (OCD) is a complex psychiatric disorder. Genetic and broad environmental factors are common risk factors for OCD. The purpose of this study is to explore the molecular mechanism of OCD and to find new molecular targets for the diagnosis and management of OCD.
Methods:
All data were downloaded from public dataset. Key modules and candidate key mRNAs were identified based on weighted gene co-expression network analysis (WGCNA). The "limma" R package was used for differential expression analysis of mRNAs. Subsequently, functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) was also carried out. In addition, a diagnostic model was constructed. Finally, the infiltration level of immune cells in OCD and its correlation with multicentric key DEmRNAs were analyzed.
Results:
Green and red modules were selected as the hub modules. A total of 447 mRNAs were considered candidate key mRNAs according to GS > 0.2 and MM > 0.3. A total of 26 DEmRNAs in the same direction were identified in the GSE60190 and GSE78104 datasets. A total of 26 DEmRNAs were intersected with candidate key mRNAs in WGCNA to obtain 10 intersection DEmRNAs (HSPB1, ITPK1, CBX7, PPP1R10, TAOK1, PISD, MKNK2, RWDD1, PPA1, and RELN). However, only four DEmRNAs (HSPB1, TAOK1, MKNK2, and PPA1) predicted related drugs. Subsequently, receiver operating characteristic analysis shows that the diagnostic model has high diagnostic value. Moreover, six multicentric key DEmRNAs (SNRPF, SNRNP70, PRPF8, NOP56, EPRS, and CCT2) were screened by UpSet package. Finally, six multicentric key DEmRNAs were found to be associated with immune cells.
Conclusion:
The key molecules obtained in this study lay a foundation for further research on the molecular mechanism of OCD.
Insights
This study identifies key molecules and a diagnostic model for obsessive-compulsive disorder (OCD). These findings advance understanding of OCD
Area of Science:
- Genetics and Molecular Biology
- Neuroscience
- Psychiatry
Background:
- Obsessive-compulsive disorder (OCD) is a complex psychiatric condition with genetic and environmental risk factors.
- Understanding the molecular underpinnings of OCD is crucial for developing effective diagnostic and therapeutic strategies.
Purpose of the Study:
- To investigate the molecular mechanisms underlying OCD.
- To identify novel molecular targets for OCD diagnosis and treatment.
Main Methods:
- Weighted gene co-expression network analysis (WGCNA) to identify key gene modules and mRNAs.
- Differential expression analysis using the "limma" R package.
- Construction of a diagnostic model and analysis of immune cell infiltration.
Main Results:
- Identified hub modules (green and red) and 447 candidate key mRNAs.
- Discovered 10 intersection differentially expressed mRNAs (DEmRNAs) between WGCNA and differential expression analysis, with four (HSPB1, TAOK1, MKNK2, PPA1) predicting related drugs.
- Developed a diagnostic model with high diagnostic value and identified six multicentric key DEmRNAs associated with immune cells.
Conclusions:
- The identified key molecules provide a foundation for further research into the molecular mechanisms of OCD.
- These findings may contribute to improved diagnostic tools and therapeutic interventions for OCD.
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