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Development and validation of ivermectin quantification method in volumetric absorptive microsampling using liquid
Yahdiana Harahap1,2, Salsabila Salsabila1, Febrina Amelia Saputri1
1Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Heliyon
|April 26, 2024
Summary
This study developed a validated method for analyzing ivermectin in blood using Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry. The new approach overcomes limitations of previous methods, offering sensitive detection of ivermectin for parasite control.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Parasitology
Background:
- Ivermectin is a crucial anthelmintic for treating onchocerciasis, but its low systemic concentration necessitates sensitive detection methods.
- Existing methods for ivermectin analysis in blood face challenges, particularly hematocrit-related effects.
- Accurate quantification of ivermectin is vital for effective parasite control and patient monitoring.
Purpose of the Study:
- To establish and validate a selective and sensitive analytical method for ivermectin in whole blood.
- To utilize doramectin as an internal standard for improved accuracy in ivermectin quantification.
- To address the limitations of previous analytical techniques, including hematocrit effects.
Main Methods:
- Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS) was employed for analysis.
- Volumetric Absorptive Microsampling (VAMS) was used for sample collection, mitigating hematocrit variability.
- Protein precipitation with acetonitrile/methanol and optimized chromatographic conditions (Acquity® UPLC BEH C18 column, isocratic elution) were utilized.
Main Results:
- The optimized method achieved sensitive detection of ivermectin using Multiple Reaction Monitoring (MRM).
- Specific m/z transitions were identified for ivermectin (892.41 > 569.5) and doramectin (916.41 > 331.35).
- The method demonstrated a Lower Limit of Quantification (LLOQ) of 1 ng/mL within a linear range of 1-150 ng/mL.
Conclusions:
- A validated UHPLC-MS/MS method for ivermectin analysis in whole blood was successfully established.
- The method, utilizing VAMS and doramectin as an internal standard, is suitable for sensitive and selective ivermectin quantification.
- This validated assay provides a reliable tool for therapeutic drug monitoring and pharmacokinetic studies of ivermectin.
