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Hangeshashinto Inhibits Porphyromonas gingivalis Pathogen-Associated Molecular Patterns-Mediated IL-6 and IL-8

Hourei Oh1, Yoshimasa Makita2, Kazuya Masuno1

  • 1Center of Innovation in Dental Education, Osaka Dental University, Osaka 573-1121, Japan.

Evidence-Based Complementary and Alternative Medicine : Ecam
|April 26, 2024
PubMed

Insights

Hangeshashinto, a traditional medicine, inhibits inflammatory responses by blocking key signaling pathways like nuclear factor-kappaB (NF-κB) activation. This study clarifies its anti-inflammatory mechanism in oral cancer cells against bacterial stimulation.

Area of Science:

  • Immunology
  • Pharmacology
  • Oral Biology

Background:

  • Hangeshashinto is known for anti-inflammatory effects, but its intracellular mechanisms are unclear.
  • Oral bacteria like Porphyromonas gingivalis can trigger inflammatory responses via pathogen-associated molecular patterns (PAMP).

Purpose of the Study:

  • To investigate the impact of hangeshashinto on intracellular signal transduction pathways in oral cancer cells stimulated by P. gingivalis PAMP.
  • To elucidate the molecular mechanisms underlying hangeshashinto's anti-inflammatory properties.

Main Methods:

  • Oral cancer cells were stimulated with P. gingivalis PAMP.
  • The effects of hangeshashinto on cytokine production (IL-6, IL-8) and promoter activation were measured.
  • Activation of Toll-like receptor (TLR) signaling and nuclear factor-kappaB (NF-κB) pathways were assessed.

Main Results:

  • Hangeshashinto inhibited P. gingivalis PAMP-induced production of interleukin-6 (IL-6) and IL-8.
  • It suppressed IL-6 promoter activation and TLR-mediated NF-κB activation.
  • These effects were comparable to known TLR inhibitors and an immunosuppressant.

Conclusions:

  • Hangeshashinto exerts anti-inflammatory effects by inhibiting TLR signal transduction pathways.
  • Suppression of NF-κB activation is a key mechanism, leading to reduced NF-κB-dependent gene expression.
  • This study provides molecular insights into hangeshashinto's anti-inflammatory action in the context of oral inflammation.

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