Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Athletic Intervention After Thoracic Surgery for Lung Cancer: Rationale and Design of the ATHENA Study.

Health science reports·2026
Same author

Andes virus-associated cardiopulmonary syndrome: diagnostic approaches, clinical management, and evidence gaps-a scoping review.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

Intragraft tacrolimus: The missing link in heart transplant acute rejection monitoring.

JHLT open·2026
Same author

When Hours Matter: A 24/7 Laboratory and Fast-Track Diagnostic Pathway for Blood Cultures in Critical Patients.

Antibiotics (Basel, Switzerland)·2026
Same author

Necrotizing Fasciitis in Northern Italy: Clinical Characteristics, Risk Factors, and Prognostic Value of the LRINEC Score-A Single-Center Retrospective Case Series.

Infectious disease reports·2026
Same author

Seven versus fourteen days for resistant bloodstream infections: what do we actually know? A scoping review.

Infection·2026

Related Experiment Video

Updated: Jun 27, 2025

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction
00:05

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

16.3K

Daptomycin Exposure Prediction With a Limited Sampling Strategy.

Simona De Gregori1, Elena Seminari2, Mara Capone1

  • 1Department of Diagnostic Medicine and Services: Laboratory Medicine-Clinical and Experimental Pharmacokinetics Unit, Fondazione IRCCS Policlinico, San Matteo, Pavia, Italy.

Therapeutic Drug Monitoring
|April 26, 2024
PubMed
Summary

A new method estimates daptomycin exposure (AUC 0-24) using just two blood samples. This limited sampling strategy aids in tailoring antibiotic doses for better treatment of Staphylococcus aureus infections.

More Related Videos

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
08:57

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Published on: October 5, 2017

10.9K
Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
08:54

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

Published on: May 19, 2020

7.7K

Related Experiment Videos

Last Updated: Jun 27, 2025

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction
00:05

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

16.3K
Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
08:57

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Published on: October 5, 2017

10.9K
Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
08:54

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

Published on: May 19, 2020

7.7K

Area of Science:

  • Pharmacokinetics and Pharmacodynamics
  • Infectious Diseases
  • Clinical Pharmacology

Background:

  • Daptomycin, a lipopeptide antibiotic, treats serious Staphylococcus aureus infections like endocarditis.
  • Therapeutic efficacy is linked to the ratio of area under the concentration-time curve (AUC 0-24) to minimum inhibitory concentration.
  • Accurate AUC 0-24 estimation is crucial for optimizing daptomycin treatment.

Purpose of the Study:

  • To develop a limited sampling strategy for estimating daptomycin's 24-hour area under the concentration-time curve (AUC 0-24).
  • To simplify the monitoring of daptomycin pharmacokinetics in clinical settings.
  • To enable personalized dosing regimens based on estimated drug exposure.

Main Methods:

  • Calculated 68 daptomycin AUC 0-24 values from 50 patients receiving at least 5 days of treatment.
  • Utilized high-performance liquid chromatography-tandem mass spectrometry for plasma concentration analysis.
  • Employed multiple regression analysis with two concentration-time points to predict AUC 0-24, assessing prediction bias and precision.

Main Results:

  • Daptomycin trough concentration and plasma levels at 2 hours post-dose effectively estimated AUC 0-24.
  • Achieved a mean prediction error of 1.6 and a mean absolute prediction error of 11.8%.
  • 73% of predicted errors fell within a clinically acceptable ±15% range.

Conclusions:

  • A validated equation allows for accurate estimation of daptomycin AUC 0-24 using limited sampling.
  • This strategy enhances clinical utility for personalized daptomycin dosing.
  • Beneficial for patients at high risk of treatment failure or delayed response.