A Preliminary Study on Quantitative Analysis of Collagen and Apoptosis Related Protein on 1064 nm Laser-Induced Skin Injury

Qiong Ma ¹, Yingwei Fan ², Yufang Cui ¹

⁰Beijing Institute of Radiation Medicine, Beijing 100850, China.

Biology +

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April 26, 2024

View abstract on PubMed

Summary

Laser irradiation of porcine skin can delay wound healing by increasing collagen deficiency and apoptosis. Medium-high laser doses lead to sluggish recovery, reduced collagen density, and elevated proapoptotic proteins.

Area Of Science

Dermatology
Biomedical Engineering
Cell Biology

Background

Laser treatments can cause skin injuries.
Understanding the healing process and molecular changes is crucial for managing laser-induced wounds.

Purpose Of The Study

To investigate factors affecting collagen and apoptosis in laser-induced porcine skin injuries.
To analyze the relationship between laser power, collagen structure, and apoptosis during wound healing.

Main Methods

Porcine skin was irradiated using a 1064 nm laser at varying power outputs.
Wound healing, collagen alterations, and apoptosis were monitored from 6 hours to 28 days post-irradiation.

Main Results

Medium-high laser doses resulted in delayed wound contraction and recovery by day 28.
These groups showed reduced collagen density and increased type III collagen.
Apoptotic cell counts and proapoptotic proteins (Bax, caspase-3, caspase-9) significantly increased between days 14 and 28.

Conclusions

Delayed wound healing in medium-high dose laser-irradiated porcine skin is associated with insufficient collagen generation.
Increased apoptosis, indicated by elevated proapoptotic proteins, contributes to impaired skin regeneration.

Keywords:

apoptosis-related protein collagen infrared laser laser-induced skin wound