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Engineering a Microphysiological Model for Regenerative Endodontic Studies.

Diana Sanz-Serrano1,2, Montse Mercade1,2, Francesc Ventura2,3

  • 1Department of Dentistry, Universitat de Barcelona, 08907 L'Hospitalet de Llobregat, Spain.

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A new 3D model of immature root canals aids endodontic research. This system showed 17% EDTA and 9% HEBP irrigating solutions effectively support dental stem cell viability and adherence.

Keywords:
apical papillacytotoxicityendodontic irrigantsmicrofluidicsstem cellsthree-dimensional culture

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dental Research

Background:

  • Dental pulp infections necessitate endodontic treatment for root canal disinfection.
  • Current in vitro models inadequately represent immature root canal anatomy, limiting regenerative endodontic research.

Purpose of the Study:

  • To develop a 3D microphysiological system (MPS) simulating immature root canals.
  • To evaluate the cytotoxicity of irrigating solutions on dental stem cells within the MPS.

Main Methods:

  • Developed a 3D MPS mimicking immature root canal anatomy.
  • Utilized Dental Stem Cells SV40 (DSCS) derived from human apical papilla stem cells.
  • Assessed irrigating solutions' effects on cell viability and adherence in 2D and 3D models.

Main Results:

  • Irrigating solutions reduced cell viability in 2D cultures and affected cell adhesion in the MPS.
  • In the 3D MPS, 17% EDTA and 9% 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) showed superior DSCS viability and adherence compared to controls.
  • Comparable efficacy was observed between 9% HEBP and 17% EDTA.

Conclusions:

  • The developed 3D MPS is a valuable tool for translational research in endodontics.
  • 9% HEBP presents a potential alternative to 17% EDTA for clinical applications in root canal treatment.