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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 28, 2026

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

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Published on: April 11, 2013

Photocell-Based Optofluidic Device for Clogging-Free Cell Transit Time Measurements.

Filippo Storti^1,2, Silvio Bonfadini¹, Gaia Bondelli^1,2

¹Centre for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milano, Italy.

|April 26, 2024

Summary

This summary is machine-generated.

This study introduces a microfluidic device using photocells to measure cell transit time through constrictions, simplifying cell deformability analysis. The novel approach offers a rapid, robust, and clog-free method for assessing cell physiology.

Keywords:

FLICE MCF-7 cancer cells cell deformability cell transit time clogging-free optofluidic transit time measurement

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Area of Science:

Biophysics
Microfluidics
Cell Biology

Background:

Cell deformability is crucial for understanding cell physiology and disease.
Current methods for measuring cell transit time are often complex and require bulky equipment.
Developing simpler, robust diagnostic tools for cell analysis is a significant challenge.

Purpose of the Study:

To develop a microfluidic device coupled with photocells for rapid and accurate measurement of cell transit time.
To demonstrate the utility of this device for assessing cell deformability.
To overcome the limitations of existing techniques for diagnostic applications.

Main Methods:

Fabrication of a monolithic 3D microfluidic device using femtosecond laser irradiation followed by chemical etching (FLICE).
Integration of photocells for optical detection of cells passing through a 3D constriction.
Measurement of transit times for breast cancer cells (MCF-7) and Latrunculin A-treated MCF-7 cells.

Main Results:

Successfully measured transit times of MCF-7 cells without complex external instrumentation.
Demonstrated a measurable difference in transit times between pristine and drug-treated cells, indicating altered deformability.
Achieved high throughput (4000-10,000 cells/min) with a clogging-free operation.

Conclusions:

The developed microfluidic-photocell device offers a simplified, robust, and high-throughput method for cell transit time measurement.
This approach facilitates the study of cell deformability and has potential for diagnostic applications.
The ease of use and lack of demanding computational efforts bring this technique closer to real-world scenarios.