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A Lateral-Flow Device for the Rapid Detection of Scedosporium Species

Genna E Davies1, Christopher R Thornton1,2

  • 1ISCA Diagnostics Ltd., B12A, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS, UK.

Diagnostics (Basel, Switzerland)
|April 26, 2024
PubMed

Insights

A new rapid lateral-flow device (LFD) test detects Scedosporium fungal infections in 30 minutes. This sensitive test uses a monoclonal antibody to identify Scedosporium-specific biomarkers in human serum, aiding in faster diagnosis.

Area of Science:

  • Medical Mycology
  • Diagnostic Microbiology
  • Immunodiagnostics

Background:

  • Scedosporium species are significant human pathogens causing diverse infections.
  • Current diagnostic methods for Scedosporium infections are slow and invasive.
  • There is a critical need for rapid and sensitive diagnostic tools for Scedosporium.

Purpose of the Study:

  • To develop a rapid and sensitive diagnostic test for Scedosporium infections.
  • To detect Scedosporium-specific biomarkers in human serum.

Main Methods:

  • Development of a lateral-flow device (LFD) test utilizing a Scedosporium-specific IgG1 monoclonal antibody (mAb), HG12.
  • The LFD targets extracellular polysaccharide (EPS) antigens secreted by Scedosporium hyphae.
  • Testing compatibility with human serum and determining limits of detection (LODs) for key Scedosporium species.

Main Results:

  • The developed Scedosporium-specific LFD (ScedLFD) test provides results in 30 minutes with pmoL/L sensitivity.
  • The test accurately detects EPS biomarkers from S. apiospermum, S. aurantiacum, and S. boydii in human serum.
  • Achieved LODs were approximately 0.81 ng/mL for S. apiospermum, 0.94 ng/mL for S. aurantiacum, and 1.95 ng/mL for S. boydii.

Conclusions:

  • The ScedLFD test offers a novel, rapid, and sensitive method for detecting Scedosporium infections.
  • This diagnostic tool has the potential to significantly improve the management of Scedosporium-related diseases.
  • The test enables the detection of multiple Scedosporium species responsible for human infections.
Keywords:
Scedosporiumbiomarkerenzyme-linked immunosorbent assaylateral-flow devicemonoclonal antibodyscedosporiosis

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