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Updated: Jun 27, 2025

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A Novel Method for Fabricating the Undulating Structures at Dermal-Epidermal Junction by Composite Molding Process.

Hao Qiao1, Chuang Gao1, Chunxiang Lu1

  • 1School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China.

Journal of Functional Biomaterials
|April 26, 2024
PubMed
Summary

Researchers developed a novel nanofiber membrane mimicking the skin's dermal-epidermal junction (DEJ). This engineered DEJ structure significantly enhanced the adhesion and proliferation of skin cells compared to flat membranes.

Keywords:
3D printingPLGA-PCL nanofiberselectrospinningmicro-imprintingmicrostructure

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Dermal-Epidermal Junction (DEJ) Simulation

Background:

  • Current tissue-engineered skin models lack the complex dermal-epidermal junction (DEJ).
  • Limitations in biomaterial properties and fabrication hinder DEJ simulation.
  • The DEJ is crucial for skin tissue function.

Purpose of the Study:

  • To engineer a nanofiber membrane that mimics the DEJ's fluctuating structure.
  • To evaluate the biocompatibility and cell proliferation effects of the engineered DEJ.

Main Methods:

  • Composite molding and electrospinning of poly(lactic-co-glycolic acid) (PLGA) and polycaprolactone (PCL) nanofibers.
  • Fabrication of a 15%PLGA + 5%PCL nanofiber membrane with optimized properties.
  • Creation of microstructures on the membrane using 3D printing and micro-imprinting.

Main Results:

  • A 15%PLGA + 5%PCL nanofiber membrane exhibited favorable mechanical properties, stability, and biocompatibility.
  • The microstructural membrane significantly promoted the adhesion and proliferation of keratinocytes (HaCaTs) and fibroblasts (HSFs).
  • Cell performance on the microstructural membrane surpassed that on a flat membrane.

Conclusions:

  • The developed nanofiber membrane effectively simulates the DEJ structure.
  • The engineered DEJ enhances skin cell adhesion and proliferation, offering a promising advancement for skin tissue engineering.