A Novel Method for Fabricating the Undulating Structures at Dermal-Epidermal Junction by Composite Molding Process.

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Dermal-Epidermal Junction (DEJ) Simulation

Background:

• Current tissue-engineered skin models lack the complex dermal-epidermal junction (DEJ).
• Limitations in biomaterial properties and fabrication hinder DEJ simulation.
• The DEJ is crucial for skin tissue function.

Purpose of the Study:

• To engineer a nanofiber membrane that mimics the DEJ's fluctuating structure.
• To evaluate the biocompatibility and cell proliferation effects of the engineered DEJ.

Main Methods:

• Composite molding and electrospinning of poly(lactic-co-glycolic acid) (PLGA) and polycaprolactone (PCL) nanofibers.
• Fabrication of a 15%PLGA + 5%PCL nanofiber membrane with optimized properties.
• Creation of microstructures on the membrane using 3D printing and micro-imprinting.

Main Results:

• A 15%PLGA + 5%PCL nanofiber membrane exhibited favorable mechanical properties, stability, and biocompatibility.
• The microstructural membrane significantly promoted the adhesion and proliferation of keratinocytes (HaCaTs) and fibroblasts (HSFs).
• Cell performance on the microstructural membrane surpassed that on a flat membrane.

Conclusions:

• The developed nanofiber membrane effectively simulates the DEJ structure.
• The engineered DEJ enhances skin cell adhesion and proliferation, offering a promising advancement for skin tissue engineering.