Integrated Process for Schizochytrium Oil Extraction, Enzymatic Modification of Lipids and Concentration of DHA Fatty Acid Esters Using Alternative Methodologies
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View abstract on PubMed
Summary
This summary is machine-generated.Marine microalgae provide a sustainable source of docosahexaenoic acid (DHA), an omega-3 fatty acid beneficial for neurodegenerative diseases. This study optimized extraction and enzymatic modification to yield purified DHA fatty acid esters from Schizochytrium sp.
Area Of Science
- Marine Biotechnology
- Nutraceutical Chemistry
- Biochemical Engineering
Background
- Marine microalgae, specifically *Schizochytrium* sp., are rich sources of docosahexaenoic acid (DHA), an omega-3 fatty acid with neuroprotective properties.
- There is a growing interest in DHA for preventing neurodegenerative diseases, necessitating sustainable and efficient production methods.
- Fish oil is a traditional source of DHA, but microalgae offer a renewable alternative.
Purpose Of The Study
- To develop an integrated process for obtaining bioactive and purified DHA fatty acid esters from *Schizochytrium* sp. using renewable resources and alternative methodologies.
- To optimize microalgal oil extraction and enzymatic modification for high DHA yield and purity.
- To explore the potential of purified DHA-FAEE as nutraceutical and pharmaceutical intermediates.
Main Methods
- Lyophilized *Schizochytrium* biomass was utilized as a DHA source.
- Pressurized liquid extraction (PLE) was optimized using a response-surface method for oil extraction.
- Enzymatic modification via ethanolysis using immobilized *Candida antarctica* B lipase (Novozym® 435) was performed.
- Open-column chromatography was employed for the purification of DHA-fatty acid ethyl esters (DHA-FAEE).
Main Results
- Optimized PLE achieved high oil yields (29.06 ± 0.12%) with significant DHA content (51.15 ± 0.72%).
- Enzymatic ethanolysis at 40 °C with 200 mg of lipase yielded 100% fatty acid ethyl ester (FAEE) in 8 hours.
- Open-column chromatography successfully produced an enriched DHA-FAEE fraction with a high concentration of 93.2 ± 1.3% DHA.
Conclusions
- A cost-effective and sustainable process for producing purified DHA-FAEE from *Schizochytrium* microalgae was established.
- The optimized method provides a viable alternative to fish oil for DHA production.
- The purified DHA-FAEE holds potential as a valuable nutraceutical and active pharmaceutical intermediate.
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