Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

12.9K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
12.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes of patients with higher-risk myelodysplastic syndromes/neoplasms treated with hypomethylating agents + venetoclax-an analysis from the International Consortium for MDS (icMDS) VALIDATE database.

Blood cancer journal·2026
Same author

The Role of Gut Microbiota in Acute Myeloid Leukemia.

Journal of clinical medicine·2026
Same author

Clonal Hematopoiesis of Indeterminate Potential (CHIP): A Model of Mutation-Driven Thromboinflammation.

Cancers·2026
Same author

Real-World Outcomes in NPM1-Mutated Acute Myeloid Leukemia: The Impact of Measurable Residual Disease on Disease Relapse.

American journal of hematology·2026
Same author

Serum Metabolomic Signatures Indicate Oxidative Membrane Lipid Remodeling in β-Thalassemia.

Metabolites·2026
Same author

Decoding Leukemic Stem Cells in AML: From Identification to Targeted Eradication.

Diseases (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 27, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.3K

Imaging Flow Cytometry: Development, Present Applications, and Future Challenges.

Savvas Dimitriadis1, Lefkothea Dova1, Ioannis Kotsianidis2

  • 1Hematology Laboratory, Unit of Molecular Biology and Translational Flow Cytometry, University Hospital of Ioannina, 45100 Ioannina, Greece.

Methods and Protocols
|April 26, 2024
PubMed
Summary
This summary is machine-generated.

Imaging flow cytometry (ImFC) merges flow analysis and microscopy for high-throughput cell imaging. Advancements in AI are crucial for analyzing its complex data, paving the way for future research and clinical applications.

Keywords:
artificial intelligenceflow cytometryimaging flow cytometryphenotype analysis

More Related Videos

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

52.3K
Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

5.1K

Related Experiment Videos

Last Updated: Jun 27, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.3K
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

52.3K
Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

5.1K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Imaging flow cytometry (ImFC) integrates flow cytometry's throughput with microscopy's imaging detail.
  • This technology enables high-throughput morphological analysis of cell populations.
  • Its development represents a significant evolution in cytometry.

Purpose of the Study:

  • To provide a historical review of imaging flow cytometry (ImFC) development.
  • To discuss current capabilities and future advancements in ImFC.
  • To highlight the challenges and potential solutions in ImFC data analysis.

Main Methods:

  • Historical perspective on the evolution of ImFC.
  • Review of current state-of-the-art ImFC technologies.
  • Exploration of data analysis challenges and the role of artificial intelligence (AI).

Main Results:

  • ImFC combines high-throughput cell analysis with detailed imaging.
  • Software and data analysis present significant implementation hurdles.
  • Artificial intelligence (AI) shows promise for advanced data interpretation in ImFC.

Conclusions:

  • ImFC is a powerful tool for cell morphology and high-throughput analysis.
  • Novel analytical tools, particularly AI, are needed to manage ImFC data complexity.
  • ImFC is poised to become essential in research and clinical diagnostics.