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Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk
Yu-Ting Wang1, Yu-Chun Lin2,3, Yi-Huei Hsieh2
1Division of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, Taipei 115021, Taiwan.
Pathogens (Basel, Switzerland)
|April 26, 2024
Summary
A new coagulase-negative Staphylococcus species, Staphylococcus hsinchuensis sp. nov. (H164T), was identified from soymilk. This novel bacterium is distinct from its closest relatives based on genetic and biochemical analyses.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Coagulase-negative staphylococci are ubiquitous bacteria with diverse ecological niches.
- Accurate identification and classification of bacterial species are crucial for understanding microbial diversity and potential impacts.
Purpose of the Study:
- To characterize a novel coagulase-negative Staphylococcus strain isolated from soymilk.
- To determine the taxonomic position of the novel strain within the Staphylococcus genus.
Main Methods:
- Comparative 16S rRNA gene sequence analysis.
- Multilocus sequence analysis (MLSA) and phylogenomic analyses.
- Determination of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH).
- Biochemical tests including substrate fermentation and enzyme activity assays.
- Analysis of cellular fatty acid composition and menaquinone profiles.
Main Results:
- The novel strain H164T belongs to the Staphylococcus genus.
- Phylogenomic analyses indicated close relatedness to Staphylococcus gallinarum, S. nepalensis, S. cohnii, and S. urealyuticus.
- ANI (<95%) and dDDH (<70%) values confirmed H164T as a distinct species.
- Fermentation profiles (d-fructose, d-maltose, d-trehalose, d-mannitol) and enzyme activities (α-glucosidase, alkaline phosphatase) differentiated H164T from related species.
- Distinct cellular fatty acid (C15:0 iso, C15:0 anteiso) and menaquinone (MK-7, MK-8) profiles were observed.
Conclusions:
- The strain H164T represents a novel species within the Staphylococcus genus.
- The proposed name for the novel species is Staphylococcus hsinchuensis sp. nov.
- The type strain is H164T (=BCRC 81404T = NBRC 116174T).
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