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Related Experiment Video

Updated: Jun 27, 2025

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
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Cryoprotectant-Mediated Cold Stress Mitigation in Litchi Flower Development: Transcriptomic and Metabolomic

Xue-Wen Zheng1, Xin-Yue Cao1, Wen-Hao Jiang1

  • 1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Metabolites
|April 26, 2024
PubMed
Summary

Bihu cryoprotectant treatment enhances litchi cold resistance by modulating gene expression and metabolite profiles, potentially increasing fruit yield. Further genomic research is recommended for cold stress mechanisms.

Keywords:
BihuLianglilow temperatureplant growth regulatorβ-alanine

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Area of Science:

  • Plant Physiology
  • Agricultural Science
  • Molecular Biology

Background:

  • Litchi (Litchi chinensis Sonn) is a valuable fruit crop susceptible to cold stress during flowering.
  • Low temperatures negatively impact litchi fruit production and quality.

Purpose of the Study:

  • To investigate the efficacy of two cryoprotectants (Bihu and Liangli) in mitigating cold stress in litchi.
  • To identify genes and metabolic pathways involved in cold resistance induced by cryoprotectant treatments.
  • To hypothesize the functional roles of identified genes in enhancing litchi's cold tolerance.

Main Methods:

  • Whole litchi plants were treated with Bihu and Liangli solutions to protect inflorescences from temperatures below 10 °C.
  • Biochemical analyses measured soluble protein, sugar, proline, antioxidant enzyme (SOD, POD, CAT), and MDA content.
  • Transcriptomic analysis identified differentially expressed genes (DEGs), gene ontology, and KEGG pathways.

Main Results:

  • Bihu treatment altered the expression of 1243 differentially expressed genes (DEGs) and was associated with 43 signal transduction pathway genes.
  • Bihu treatment affected 422 low-temperature-sensitive differentially accumulated metabolites (DAMs), primarily in lipid metabolism, organic oxidants, and alcohol pathways.
  • Bihu treatment showed potential to improve litchi traits and fruit productivity compared to Liangli and control treatments.

Conclusions:

  • Bihu cryoprotectant treatment confers cold resistance in litchi by modulating gene expression and metabolite profiles.
  • Identified genes and metabolic pathways, including β-alanine, polycyclic aromatic hydrocarbon, linoleic acid, and histidine metabolism, are crucial for cold tolerance.
  • Further genomic research is needed to fully elucidate the cold stress response mechanisms in litchi.