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FuncPEP v2.0: An Updated Database of Functional Short Peptides Translated from Non-Coding RNAs.

Swati Mohapatra1,2, Anik Banerjee2,3, Paola Rausseo1,4

  • 1Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Non-Coding RNA
|April 26, 2024
PubMed
Summary
This summary is machine-generated.

Functional peptides translated from non-coding RNAs (ncRNAs), termed ncPEPs, are increasingly discovered. The updated FuncPEP v2.0 database now includes 152 ncPEPs, highlighting their bivalent biological roles.

Keywords:
immunitymicro-peptidesncRNA-encoded peptidesnon-coding RNAs

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Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Non-coding RNAs (ncRNAs) are increasingly recognized for their roles beyond structural or regulatory functions.
  • Short functional peptides (ncPEPs) can be translated from ncRNAs, presenting a bivalent function for these transcripts.
  • Understanding ncPEPs is crucial for advancing biological and disease research.

Purpose of the Study:

  • To update the FuncPEP database with newly identified functional peptides translated from ncRNAs (ncPEPs).
  • To categorize host ncRNAs and analyze characteristics of newly discovered ncPEPs.
  • To provide a comprehensive resource for researchers studying ncPEPs and their functions.

Main Methods:

  • A systematic PubMed search was conducted from August 2020 to July 2023 using specific keywords.
  • Publications reporting validated functional peptides derived from ncRNAs were screened.
  • Data from identified ncPEPs were compiled and categorized, including host ncRNA type, species, identification methods, and functional distribution.

Main Results:

  • The FuncPEP v2.0 database contains 152 functional ncPEPs, with 40 novel entries identified in the last three years.
  • A steady, rather than significant, increase in newly discovered functional ncPEPs was observed.
  • Newly identified ncPEPs were characterized across various parameters including host ncRNA, species, length, identification methods, and functional roles.

Conclusions:

  • The updated FuncPEP v2.0 database expands the knowledge of functional peptides translated from ncRNAs.
  • While mass spectrometry facilitates ncPEP identification, challenges remain in fully characterizing their functionality.
  • Further research is needed to overcome limitations in proving the functional significance of identified ncPEPs.