Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Kinase-Glo luminescent kinase assay for in vitro determination of PKA activity].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2012
Same author

Functional characterization of an arrestin gene on insecticide resistance of Culex pipiens pallens.

Parasites & vectors·2012
Same author

MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms.

Experimental cell research·2012
Same author

Let-7b inhibits human cancer phenotype by targeting cytochrome P450 epoxygenase 2J2.

PloS one·2012
Same author

Role of IKK/NF-κB signaling in extinction of conditioned place aversion memory in rats.

PloS one·2012
Same author

Inhibition of poly(ADP-ribose) polymerase attenuates acute kidney injury in sodium taurocholate-induced acute pancreatitis in rats.

Pancreas·2012

Related Experiment Video

Updated: Jun 27, 2025

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.4K

Relationship between arginine methylation and vascular calcification.

Chen Chen1, Yuanyuan Ding2, Qun Huang1

  • 1Department of Nephrology, Shengjing Hospital, China Medical University, China.

Cellular Signalling
|April 26, 2024
PubMed
Summary

Arginine methylation regulates vascular calcification (VC) in chronic kidney disease patients on maintenance hemodialysis. This epigenetic process impacts vascular smooth muscle cell transdifferentiation and cardiovascular disease risk.

Keywords:
Arginine methylationChronic kidney diseaseEpigenetics modificationProtein arginine methyltransferases (PRMTs)Signaling pathwaysVascular calcification

More Related Videos

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

19.5K
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.0K

Related Experiment Videos

Last Updated: Jun 27, 2025

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.4K
Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

19.5K
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.0K

Area of Science:

  • Nephrology
  • Cardiovascular Biology
  • Epigenetics

Background:

  • Vascular calcification (VC) is a key predictor of cardiovascular disease (CVD) and mortality in maintenance hemodialysis (MHD) patients with chronic kidney disease (CKD).
  • Vascular smooth muscle cells (VSMCs) undergo osteogenic transdifferentiation, driven by oxidative stress and mineral imbalance, leading to VC.
  • Post-translational modifications, including arginine methylation, are emerging regulators of VC, similar to their role in osteoblast differentiation.

Purpose of the Study:

  • To propose that arginine methylation is a critical regulator of vascular calcification (VC) in chronic kidney disease (CKD).
  • To elucidate the molecular mechanisms by which arginine methylation influences VC through various signaling pathways.

Main Methods:

  • Review and synthesis of existing literature on vascular calcification, epigenetics, and arginine methylation in CKD.
  • Analysis of proposed signaling pathways (NF-κB, WNT, AKT/PI3K, TGF-β/BMP/SMAD, IL-6/STAT3) involved in VC regulation.
  • Examination of arginine methylation's potential impact on VC-related factors, oxidative stress, and endoplasmic reticulum stress.

Main Results:

  • Arginine methylation is proposed to regulate VSMC osteogenic transdifferentiation, a key process in VC.
  • Arginine methylation influences multiple signaling pathways implicated in VC pathogenesis.
  • This epigenetic modification may also modulate VC-associated calcification factors and cellular stress responses.

Conclusions:

  • Arginine methylation plays a significant role in regulating vascular calcification (VC) in CKD.
  • Understanding these regulatory mechanisms offers potential therapeutic targets for preventing VC and CVD in MHD patients.
  • Further research into arginine methylation's role in VC is warranted.