Global DNA Methylation Level in Tumour and Margin Samples in Relation to Human Papilloma Virus and Epstein-Barr Virus in Patients with Oropharyngeal and Oral Squamous Cell Carcinomas
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View abstract on PubMed
Summary
This summary is machine-generated.Aberrant DNA methylation is linked to cancer progression in oropharyngeal and oral squamous cell carcinoma. Global DNA methylation levels correlate with HPV/EBV coinfection, tumor grade, and patient demographics, impacting cancer prognosis.
Area Of Science
- Oncology
- Epigenetics
- Virology
Background
- Aberrant DNA methylation is a hallmark of various cancers, including oropharyngeal squamous cell carcinoma (OPSCC) and oral squamous cell carcinoma (OSCC).
- Understanding methylation patterns is crucial for improving cancer therapy and patient prognosis.
Purpose Of The Study
- To investigate the relationship between global DNA methylation levels and viral coinfections (HPV, EBV) in OPSCC and OSCC.
- To explore correlations between DNA methylation and clinical/demographical factors in these cancers.
Main Methods
- Global DNA methylation levels were analyzed using enzyme-linked immunosorbent assay (ELISA).
- Human Papillomavirus (HPV) and Epstein-Barr Virus (EBV) detection were performed using hybridization and real-time PCR, respectively.
Main Results
- Lower global DNA methylation was observed in OPSCC tumors from women compared to men (p=0.049).
- OPSCC margin samples with HPV/EBV coinfection showed reduced methylation (p=0.042).
- OSCC G3 tumors and HPV-positive OSCC tumors exhibited significantly lower methylation levels (p=0.010, p=0.013).
- A negative correlation was found between DNA methylation and N stage in OSCC (rS=-0.33, p=0.039).
- HPV-positive OPSCC patients had higher methylation than HPV-positive OSCC patients (p=0.015).
Conclusions
- DNA methylation patterns are influenced by viral factors like HPV and EBV.
- Clinical and demographical parameters also modulate DNA methylation in OPSCC and OSCC, affecting cancer prognosis.
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