Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalized polygenic profiling based on the genetic architecture of lipid metabolism in the Russian population.

Frontiers in cardiovascular medicine·2026
Same author

DrugForm-TAS: Target-Agnostic Selectivity as Proteome-wide Binding Propensity Estimation.

Computational and structural biotechnology journal·2026
Same author

Targeted Epigenetic Activation of <i>CTLA4</i> in Haploinsufficiency Cellular Models by CRISPRa.

Biomedicines·2026
Same author

Sleep Maintenance Insomnia in Older Adults: Cardiometabolic Comorbidities and Evidence of Antiviral Pathways Activation from Blood Transcriptome and dsRNA Expression Analyses.

International journal of molecular sciences·2026
Same author

Clinical, social, molecular, and genetic predictors of cognitive resilience in long-living adults without dementia.

Frontiers in dementia·2026
Same author

Longevity vs. Healthy Longevity: Different Outcomes Underlain by Different Mechanisms.

Aging and disease·2026

Related Experiment Video

Updated: Jun 27, 2025

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
16:20

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Published on: July 2, 2018

18.6K

Precisely Printable Silk Fibroin/Carboxymethyl Cellulose/Alginate Bioink for 3D Printing.

Yuliya Nashchekina1, Anastasia Militsina2, Vladimir Elokhovskiy3

  • 1Institute of Cytology of the Russian Academy of Sciences, Center of Cell Technologies, St. Petersburg 194064, Russia.

Polymers
|April 27, 2024
PubMed
Summary

Researchers developed improved silk fibroin bioinks for 3D bioprinting by adding carboxymethyl cellulose and sodium alginate. These new inks enhance printability and scaffold stability for tissue engineering applications.

Keywords:
3D printingcarboxymethyl cellulose sodiummesenchymal stromal cellssilk fibroinsodium alginate

More Related Videos

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
06:29

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Published on: May 2, 2020

6.5K
Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
09:32

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

Published on: May 11, 2021

3.2K

Related Experiment Videos

Last Updated: Jun 27, 2025

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
16:20

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Published on: July 2, 2018

18.6K
Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
06:29

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Published on: May 2, 2020

6.5K
Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
09:32

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture

Published on: May 11, 2021

3.2K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Bioprinting Technology

Background:

  • Three-dimensional (3D) bioprinting offers advanced tissue engineering capabilities by mimicking the extracellular matrix.
  • Natural polymers like silk fibroin present challenges in 3D bioprinting due to low viscosity and poor printability.

Purpose of the Study:

  • To develop highly viscous, stable, and biocompatible silk fibroin-based bioinks for improved 3D bioprinting.
  • To enhance the printability, stability, and mechanical properties of silk fibroin scaffolds.

Main Methods:

  • Formulating silk fibroin solutions with varying concentrations (2.5-5%) combined with carboxymethyl cellulose sodium (2%) and sodium alginate (1%).
  • Assessing the printability, viscosity, stability, and mechanical properties of the developed bioinks.
  • Evaluating the biocompatibility of the printed scaffolds with mesenchymal stromal cells.
  • Stabilizing printed silk fibroin scaffolds using methanol or ethanol treatment to induce beta-sheet formation.

Main Results:

  • The addition of carboxymethyl cellulose and sodium alginate significantly improved the printability, stability, and mechanical properties of silk fibroin bioinks.
  • Higher concentrations of silk fibroin correlated with enhanced printability.
  • The developed bioinks demonstrated ease of printing, high printing quality, and good biocompatibility with mesenchymal stromal cells.
  • Printed scaffolds exhibited satisfactory mechanical characteristics after stabilization.

Conclusions:

  • The novel silk fibroin-based bioinks incorporating carboxymethyl cellulose and sodium alginate overcome limitations of natural polymers in 3D bioprinting.
  • These bioinks provide a promising platform for fabricating stable, biocompatible, and mechanically sound tissue-like structures.
  • The developed bioink composition is suitable for advanced applications in tissue engineering and regenerative medicine.