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Updated: Jun 27, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Viroids of the Mediterranean Basin
Maria Kaponi1, Panayota E Kyriakopoulou2, Ahmed Hadidi3
1Plant Virology Laboratory, Benaki Phytopathological Institute, Stefanou Delta 8, Kifissia, 14561 Athens, Greece.
Mediterranean countries have made significant progress in viroid research, detecting 29 species using advanced methods and implementing effective eradication strategies. These efforts align with European and Mediterranean Plant Protection Organization (EPPO) recommendations.
Area of Science:
- Plant Pathology
- Virology
- Agricultural Science
Background:
- The Mediterranean Basin harbors 29 viroid species from the Pospiviroidae and Avsunviroidae genera.
- The region's climate and socio-cultural factors historically favor viroid transmission.
Purpose of the Study:
- To review advancements in viroid detection and eradication in Mediterranean countries.
- To highlight the application of modern molecular techniques in viroid research.
Main Methods:
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
- Next-generation sequencing (NGS)
- Molecular hybridization techniques
Main Results:
- Successful detection, identification, and characterization of known and novel viroids.
- Efficient elimination of specific viroids through EPPO-guided eradication measures.
Conclusions:
- Mediterranean countries show substantial progress in viroid research and management.
- Advanced molecular tools and strategic eradication are key to controlling viroid diseases.
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