Patient-Derived Xenograft Models for Ovarian Cancer

Trang Minh Tran ^1,2, Gwo Yaw Ho ^2,3, Simon Chu ⁴

⁰Hudson Institute of Medical Research, Clayton, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.) +

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April 27, 2024

View abstract on PubMed

Summary

Patient-derived xenograft (PDX) models are vital for in vivo research, preserving tumor characteristics and microenvironments. This chapter details four transplantation methods for developing ovarian cancer PDX models.

Area Of Science

Oncology
Translational Research
Animal Models

Background

Patient-derived xenograft (PDX) models are essential for in vivo cancer research.
PDX models retain original tumor molecular profiles and mimic the human tumor microenvironment.
In vitro models cannot fully replicate the complex in vivo tumor microenvironment.

Purpose Of The Study

To describe established methods for developing ovarian cancer patient-derived xenograft (PDX) models.
To provide a comprehensive overview of PDX model development techniques for ovarian cancer research.

Main Methods

The chapter details four distinct transplantation techniques for PDX model development.
Methods include intra-bursal, intrarenal capsule, intraperitoneal, and subcutaneous transplantation.
These methods are applied to establish ovarian cancer PDX models.

Main Results

The described methods facilitate the successful development of ovarian cancer PDX models.
PDX models generated using these techniques preserve key tumor characteristics.
Accurate tumor microenvironment replication is achieved in these models.

Conclusions

Patient-derived xenograft (PDX) models are superior to in vitro models for ovarian cancer research.
The four described transplantation methods offer viable approaches for PDX model development.
PDX models are critical for advancing ovarian cancer research and therapeutic development.

Keywords:

Intra-bursal Intraperitoneal Intrarenal capsule Ovarian cancer Patient-derived xenograft (PDX) Subcutaneous