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Published on: August 15, 2018
Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex
Pedro Trevizan-Baú1, Mitchell Ty Ringuet2, Martin J Stebbing3
1The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia; Department of Physiological Sciences, University of Florida, Gainesville, FL, USA.
Abstract:
Neurons that originate from pre-vertebral sympathetic ganglia, the splanchnic-celiac-superior mesenteric ganglion complex (SCSMG) in mouse, have important roles in control of organs of the upper abdomen. Here, we present a protocol for the isolation of the mouse sympathetic SCSMG. We describe steps for surgical incision, ganglia isolation, ganglia fine dissection, and whole-mount SCSMG after clearing-enhanced 3D (Ce3D) clearing method and immunohistochemistry. Given the importance of mice in studies of that control, this protocol aims to assist biomedical researchers in the dissection of the mouse SCSMG.
