Bifunctional enzyme-mimicking metal-organic frameworks for sensitive acetylcholine analysis

Area of Science:

• Nanomaterials Science
• Biotechnology
• Analytical Chemistry

Background:

• Multi-enzyme biosensing systems face challenges like enzyme cross-talk and high costs.
• Nanomaterials with multi-enzyme-like activity offer a solution to simplify biosensing.
• Metal-organic frameworks (MOFs) are promising platforms for developing such catalysts.

Purpose of the Study:

• To develop a novel composite catalyst with dual enzyme-like activity (AChE and POD) using MOF-808 and Pt NPs.
• To construct a simplified biosensor for acetylcholine (ACh) detection by replacing natural enzymes with the composite catalyst.
• To leverage pH variations for signal amplification and to eliminate enzyme cross-talk in the biosensing process.

Main Methods:

• Synthesized Pt nanoparticles (Pt NPs) supported on Zr-based MOF-808, creating MOF-808/Pt NPs.
• Utilized the dual enzyme-like activity of MOF-808/Pt NPs to catalyze ACh hydrolysis and subsequent substrate oxidation.
• Designed a colorimetric detection strategy based on pH-modulated POD-like activity for signal amplification.

Main Results:

• The MOF-808/Pt NPs composite successfully mimicked acetylcholinesterase and peroxidase activities.
• A biosensor for ACh detection was constructed using MOF-808/Pt NPs, simplifying the assay.
• The biosensor demonstrated signal amplification through pH-dependent activity modulation, effectively eliminating enzyme cross-talk.

Conclusions:

• MOF-808/Pt NPs offer a promising platform for developing advanced multi-enzyme-like catalysts.
• The developed biosensor provides a simplified, cost-effective, and highly sensitive method for ACh detection.
• This approach has significant potential for clinical diagnostics and other analytical applications.