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Related Experiment Video

Updated: Jun 27, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Probing RNA structure and dynamics using nanopore and next generation sequencing.

Emma Bose¹, Shengwei Xiong¹, Alisha N Jones¹

¹Department of Chemistry, New York University, New York, New York, USA.

The Journal of Biological Chemistry

|April 27, 2024

Summary

This summary is machine-generated.

RNA conformational dynamics are crucial for biological regulation. Sequencing-based methods offer a powerful alternative to study these dynamic RNA structures across diverse classes.

Keywords:

HIV RNA RNA dynamics RNA structure SARS-CoV-2 chemical probing long noncoding RNA next generation sequencing oxford nanopore read deconvolution riboswitch

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Published on: February 2, 2024

Area of Science:

Molecular Biology
Biophysics
Genomics

Background:

RNA molecules exhibit dynamic conformational changes essential for biological functions.
Traditional biophysical methods face limitations in studying diverse RNA structures due to size and flexibility.
Understanding RNA conformational dynamics is key to deciphering gene regulation and molecular interactions.

Purpose of the Study:

To provide a methodological overview of sequencing-based techniques for studying RNA conformational dynamics.
To highlight how these techniques advance the understanding of RNA structural diversity.
To illustrate the impact of these methods on RNA structure research.

Main Methods:

Integration of chemical probing experiments with next-generation sequencing (NGS).
Application of direct sequencing technologies for RNA structure analysis.
Review of various sequencing-based approaches to map RNA conformational landscapes.

Main Results:

Sequencing-based techniques overcome limitations of traditional methods for studying RNA dynamics.
These methods enable comprehensive analysis of conformational states across various RNA classes.
Demonstration of how these techniques provide new insights into RNA structural plasticity.

Conclusions:

Sequencing-based approaches represent a significant advancement in studying RNA conformational dynamics.
These methods offer broader applicability to diverse RNA types, enhancing our understanding of their biological roles.
The reviewed techniques are reshaping the paradigm of RNA structure-function relationship studies.