Related Experiment Video

Updated: Jun 27, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.6K

circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation

Zhongxin Yan1, Changling Duan2, Xi Li2

  • 1Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. yan1984@126.com.

Cancer Cell International
|April 27, 2024
PubMed
Abstract

Insights

Circular RNA TFRC (circ-TFRC) promotes ovarian cancer (OC) progression and metastasis. Downregulating circ-TFRC inhibits OC cell proliferation and migration by regulating IGF2 expression and sponging miR-615-3p.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Ovarian cancer (OC) is a significant global health concern for females.
  • Circular RNAs (circRNAs) are involved in various biological processes.
  • The role of circ-TFRC in OC progression was previously unclear.

Purpose of the Study:

  • To elucidate the regulatory mechanism of circ-TFRC in ovarian cancer.
  • To investigate the role of circ-TFRC in OC progression and metastasis.

Main Methods:

  • Luciferase reporter assay to identify downstream targets.
  • Transwell migration, EdU, and CCK-8 assays for proliferation and migration.
  • In vivo tumorigenesis and metastasis assays in OC models.

Main Results:

  • circ-TFRC expression was elevated in OC cells and tissues.
  • circ-TFRC downregulation suppressed OC cell proliferation and migration in vitro and in vivo.
  • miR-615-3p and IGF2 were identified as direct downstream targets of circ-TFRC.
  • IGF2 overexpression or miR-615-3p inhibition counteracted the effects of circ-TFRC silencing.

Conclusions:

  • circ-TFRC downregulation inhibits ovarian cancer progression and metastasis.
  • The mechanism involves the regulation of IGF2 expression and sponging of miR-615-3p by circ-TFRC.
Keywords:
IGF2Ovarian cancercirc-TFRCmiR-615-3p

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.7K
A Proximal Culture Method to Study Paracrine Signaling Between Cells08:17

A Proximal Culture Method to Study Paracrine Signaling Between Cells

Published on: August 28, 2018

9.5K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K