Related Experiment Video

Updated: Jun 27, 2025

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer
07:55

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

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Tumor cells inhibit the activation of ILC2s through up-regulating PD-1 expression

Chaoyun Yin1,2, Yani Pa2, Guangyu Li2

  • 1Department of Emergency Medicine, The Affiliated Jiangyin Hospital of Nantong University, Jiangyin, P. R. China.

Immunopharmacology and Immunotoxicology
|April 28, 2024
PubMed
Abstract

Insights

Programmed cell death-1 (PD-1) up-regulation inhibits innate lymphoid cells (ILC2s) in lung cancer. Blocking PD-1 with anti-PD-1 therapy restores ILC2 function and suppresses tumor growth, offering a new immunotherapy strategy.

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Programmed cell death-1 (PD-1) and programmed cell death ligand-1 (PD-L1) interactions on immune cells can hinder anti-tumor responses.
  • Understanding these interactions is crucial for improving cancer immunotherapy efficacy.

Purpose of the Study:

  • To investigate the role of PD-1 expression in lung cancer-infiltrating type II innate lymphoid cells (ILC2s).
  • To explore the potential of targeting the PD-1/PD-L1 axis for enhancing ILC2-based cancer immunotherapy.

Main Methods:

  • Analysis of PD-1 expression in lung cancer-infiltrating ILC2s.
  • In vitro co-culture experiments with tumor cells and anti-PD-1 treatment.
  • In vivo studies using LLC-bearing nude mice treated with anti-PD-1.

Main Results:

  • PD-1 was highly up-regulated on lung cancer-infiltrating ILC2s, suppressing their activation and function.
  • Anti-PD-1 treatment restored ILC2 cytokine secretion and anti-tumor activity in vitro.
  • In vivo anti-PD-1 therapy promoted ILC2 activation and inhibited tumor growth.

Conclusions:

  • A novel PD-1/PD-L1 regulatory mechanism involving innate immune cells was identified.
  • Targeting this axis offers a promising strategy for ILC2-based immunotherapy in cancer treatment.
Keywords:
ILC2sLung cancerPD-1PD-l1cancer microenvironment

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