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Published on: May 26, 2023
Protocol to measure monosynaptic connections between different cortical regions in mice using cell-pair cross
Cong Wang1, Montana Samantzis2, Matilde Balbi2
1Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai 201203, China; Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
Abstract:
Neuromodulation can facilitate interactions between neurons to rescue impaired brain function after stroke. Here, we present a protocol for measuring putative monosynaptic connections between different cortical regions. We detail procedures for tetrode fabrication, implantation surgery, stroke induction in mice, multi-site in vivo electrophysiological recording, units clustering, principal neuron/interneuron classification, and functional connection analysis. This protocol allows us to understand the mechanisms of stroke recovery. For complete details on the use and execution of this protocol, please refer to Wang et al.1.
