Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Downstream Processing

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Admission Cytokine Profiling for ICU Mortality Prediction in Heterogeneous Acute Respiratory Failure: An Exploratory Cytokine Profiling Study.

Diagnostics (Basel, Switzerland)·2026

Same author

Genome-guided discovery of antibiotic activity in <i>Streptomyces virginiae</i> THA-960 against multidrug resistant <i>Staphylococcus aureus</i>.

iScience·2026

Same author

Dihydropyridine Calcium Channel Blocker-induced Prescribing Cascades: Signal Detection using High-throughput Sequence Symmetry Analysis.

medRxiv : the preprint server for health sciences·2026

Same author

FuNGI: Fungal Nucleolar Genomic Inventory-a comprehensive database of fungal proteins with predicted nucleolar localization signals.

Database : the journal of biological databases and curation·2026

Same author

Building a credibility-based framework for target discovery: Perspectives from tRNA synthetase-linked metabolic diseases.

Briefings in bioinformatics·2026

Same author

Parametric study on electro-coagulation for efficient removal of microplastics from reverse osmosis wastewater.

Water science and technology : a journal of the International Association on Water Pollution Research·2026

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Published on: June 22, 2012

Process optimization for microfluidic preparation of liposomes using food-grade components.

Sung-Chul Hong¹, Chi Rac Hong², Minsoo Kim³

¹Department of Food Science and Biotechnology, Kunsan National University, Gunsan 54150, Republic of Korea.

|April 28, 2024

Summary

This study developed optimized food-grade liposomes for the food industry. These liposomes match conventional ones in properties, ensuring safety and effectiveness for delivery systems.

Keywords:

Food-grade component Liposome Microfluidizer Plackett–Burman design Process optimization Response surface methodology

More Related Videos

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Published on: June 22, 2012

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Area of Science:

Food Science and Technology
Materials Science
Chemical Engineering

Background:

Liposomes are versatile delivery systems.
Conventional liposome production often uses non-food-grade materials.
There is a need for safe, sustainable liposome manufacturing for the food industry.

Purpose of the Study:

To optimize a sustainable liposome manufacturing process using food-grade components.
To compare the properties of food-grade liposomes with conventionally produced ones.
To evaluate the suitability of food-grade liposomes for food industry applications.

Main Methods:

Liposomes were prepared using food-grade solvents and ingredients.
Physicochemical, morphological, and interfacial properties were analyzed.
Plackett-Burman design and response surface methodology were employed for optimization.
Key formulation parameters including soy lecithin, β-sitosterol, and water-in-oil (W/O) ratio were identified.

Main Results:

No significant differences in particle size (~196 nm) and ζ-potential (~-45 mV) were observed between food-grade and conventional liposomes.
Optimized manufacturing parameters were determined: 3.17 g soy lecithin, 0.25 g β-sitosterol, and a 1:2.59 W/O ratio.
The optimized process yielded stable liposomes with desirable characteristics.

Conclusions:

Sustainable liposome production using food-grade materials is feasible.
Food-grade liposomes possess comparable properties to conventional ones.
This advancement supports the safe and effective use of liposomes in the food industry, meeting regulatory standards.