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M2 Macrophage Prominently Distributed in the Rat's Colon of DMH-Induced Inflammation Associated Colorectal Cancer
Windy Arensya Omadhika1, Solikhah Solikhah1, Albertus Ari Adrianto2
1Faculty of Biology Universitas Gadjah Mada, Yogyakarta, Indonesia.
Objective:
The aim of this study is to examine the M1 and M2 macrophages distribution in the rat's colon of DMH-induced inflammation associated colorectal cancer.
Methods:
Colon tissue of three groups of 4 rats that induced using 1,2 dimethylhydrazine (DMH) at 30 mg/kg bw every week for 9, 11, and 13 weeks were used. The M1 and M2 distribution was examined by using antibody anti iNOS for M1 and anti-CD163 for M2 with immunohistochemistry method. The data was presents in figure and table in the form of percentage.
Result:
M1 macrophage was found in all groups in the low distribution level (25% - 50%), while M2 macrophage was observed in all groups with 100% distribution. In the longer period of DMH induction, M2 macrophages was distributed more abundant.
Conclusion:
All of the rat's colon showing chronic inflammation that led to the tumorigenesis.
