Optical Analysis Method for Turbid Media Based on Wedge-Shaped Cells at Different Angles

Area of Science:

• Optical Physics
• Analytical Chemistry

Background:

• Spectral imaging of turbid media is crucial for component analysis.
• Flat sample cells offer limited scattering information, impacting detection accuracy.

Purpose of the Study:

• To model and analyze the influence of wedge angles on turbid medium component detection precision.
• To optimize the accuracy of spectral imaging analysis using wedge-shaped sample cells.

Main Methods:

• Investigated incident angles from 10° to 45° with a 5° gradient.
• Utilized wedge-shaped sample cells with varying wedge angles.
• Performed validation experiments with Indian ink and fat emulsion solutions.
• Employed partial least-squares regression (PLSR) for optical parameter analysis.

Main Results:

• A 35° incident angle in the wedge-shaped cell model yielded optimal analysis results.
• Achieved a highest R² of 0.980 and RMSEP of 0.002 with the optimal model.
• The wedge-shaped cell model showed improved accuracy (R² increased by 0.041, RMSEP decreased by 0.004) compared to flat cells.

Conclusions:

• Wedge-shaped sample cells significantly enhance spectral image information content for turbid media.
• Optimizing incident angles, particularly at 35°, refines detection precision for turbid medium components.
• This study provides a foundation for advanced turbid medium analysis using wedge-shaped sample cells.