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Optical Analysis Method for Turbid Media Based on Wedge-Shaped Cells at Different Angles.

Zhe Zhao^1,2, Ruina Zhu^3,2, Yuyan Yang^1,2

¹School of Electronics and Information Engineering, Tiangong University, Tianjin 300387, China.

|April 29, 2024

Summary

Wedge-shaped sample cells improve turbid media analysis. Optimal detection precision for turbid medium components was achieved using a 35° incident angle, enhancing spectral imaging accuracy.

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Area of Science:

Optical Physics
Analytical Chemistry

Background:

Spectral imaging of turbid media is crucial for component analysis.
Flat sample cells offer limited scattering information, impacting detection accuracy.

Purpose of the Study:

To model and analyze the influence of wedge angles on turbid medium component detection precision.
To optimize the accuracy of spectral imaging analysis using wedge-shaped sample cells.

Main Methods:

Investigated incident angles from 10° to 45° with a 5° gradient.
Utilized wedge-shaped sample cells with varying wedge angles.
Performed validation experiments with Indian ink and fat emulsion solutions.
Employed partial least-squares regression (PLSR) for optical parameter analysis.

Main Results:

A 35° incident angle in the wedge-shaped cell model yielded optimal analysis results.
Achieved a highest R² of 0.980 and RMSEP of 0.002 with the optimal model.
The wedge-shaped cell model showed improved accuracy (R² increased by 0.041, RMSEP decreased by 0.004) compared to flat cells.

Conclusions:

Wedge-shaped sample cells significantly enhance spectral image information content for turbid media.
Optimizing incident angles, particularly at 35°, refines detection precision for turbid medium components.
This study provides a foundation for advanced turbid medium analysis using wedge-shaped sample cells.