Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-situ cascade assembled peptide-drug conjugate for the treatment of bladder cancer by enhancing membrane-entry and lysosome destabilization.

Biomaterials·2026
Same author

Impact of Treadmill Running Intensity on Lubricin Levels in Rat Achilles Tendons.

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca·2026
Same author

Metabolism-modulating nanoparticles for remodeling adipose-liver crosstalk to reverse liver fibrosis.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Terpenoids from <i>Euphorbia ebracteolata</i> (Hayata.) with their multidrug resistance reversal activity.

Natural product research·2026
Same author

Wen Yang Ding Chuan Tang Attenuates Asthmatic Airway Remodeling by Inhibiting M2 Macrophage-Driven Fibroblast Activation.

Journal of visualized experiments : JoVE·2026
Same author

Nanotherapy targeting liver sinusoidal endothelial cells in liver fibrosis.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Orthopedic trinity: Arthroscopy, sports medicine, and regenerative orthopedics.

World journal of orthopedics·2026
Same journal

Laminectomy alone <i>vs</i> laminectomy with posterior fusion in lumbar spinal stenosis: The role of instability.

World journal of orthopedics·2026
Same journal

Early dislocation in primary total hip arthroplasty: Evaluation of treatment options following closed reduction.

World journal of orthopedics·2026
Same journal

Phosphatidylinositol-3-kinase/protein kinase B signaling dysregulation in steroid-induced osteonecrosis of the femoral head: A minireview of therapeutic implications.

World journal of orthopedics·2026
Same journal

Impact of preoperative frailty on complications, readmissions, and functional recovery following total knee arthroplasty in elderly patients.

World journal of orthopedics·2026
Same journal

Weight-adjusted low molecular weight heparin with surgery morning dose administration: Impact on hip hemiarthroplasty early infections.

World journal of orthopedics·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2025

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.0K

Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis.

Xu-Chang Zhou1, Dong-Xue Wang1, Chun-Yu Zhang1

  • 1School of Sport Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China.

World Journal of Orthopedics
|April 29, 2024
PubMed
Summary
This summary is machine-generated.

Regular treadmill exercise in growing mice boosts bone density and formation. The study reveals the lncRNA H19/miR-149 axis is crucial for osteogenic differentiation and bone development.

Keywords:
AutophagyBone formationBone mesenchymal stem cellsExerciseOsteogenic differentiation

More Related Videos

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.6K

Related Experiment Videos

Last Updated: Jun 27, 2025

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.0K
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.6K

Area of Science:

  • Bone Biology and Exercise Physiology
  • Molecular Mechanisms of Osteogenesis

Background:

  • Childhood and adolescent physical activity is vital for bone development, increasing bone density and peak bone mass.
  • Exercise stimulates bone formation through enhanced bone development.

Purpose of the Study:

  • To investigate the effects of exercise on bone formation in growing mice.
  • To elucidate the underlying molecular mechanisms, focusing on the lncRNA H19/miR-149 axis.

Main Methods:

  • Growing mice were divided into control and treadmill exercise groups.
  • Micro-CT scanning, immunohistochemistry, RT-qPCR, and Western Blot were employed.
  • Bioinformatics analysis identified potential targets of long non-coding RNA H19 (lncRNA H19).

Main Results:

  • Treadmill exercise significantly increased bone mineral density, bone volume, and trabecular number in mouse femurs.
  • Inhibition of lncRNA H19 upregulated miR-149 and suppressed osteogenic differentiation markers.
  • lncRNA H19 knockdown downregulated autophagy markers, indicating a role in regulating osteogenic processes.

Conclusions:

  • Moderate treadmill exercise effectively stimulates bone formation, enhancing bone density and volume in growing mice.
  • The lncRNA H19/miR-149 axis is a key regulator of osteogenic differentiation.
  • Exercise-induced bone benefits are mediated by molecular pathways involving lncRNA H19.