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Updated: Jun 27, 2025
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Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Published on: April 21, 2016
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Engineering peptide-modified alginate-based bioinks with cell-adhesive properties for biofabrication
Emine Karakaya1, Luisa Gleichauf1, Lisa Schöbel1
1Department of Material Science and Engineering, Institute for Biomaterials, Friedrich-Alexander University Erlangen-Nuremberg Germany rainer.detsch@fau.de.
RSC Advances
|April 29, 2024
Summary
This study modified alginate (ALG) and alginate-dialdehyde (ADA) bioinks with cell-adhesive peptides for 3D bioprinting. Peptide combinations enhanced cell spreading, showing promise for improved biomaterial interactions.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Bioprinting Technology
Background:
- Alginate (ALG) and alginate-dialdehyde (ADA) are key hydrogel materials for 3D bioprinting and drop-on-demand (DoD) applications.
- Enhancing cell-material interactions requires modifying these polymers with cell-adhesive peptide sequences to create effective bioinks.
Purpose of the Study:
- To explore the modification of ALG- and ADA-based bioinks with specific adhesive peptides (YIGSR, RRETEWA, IKVAV).
- To evaluate the impact of different coupling methods (carbodiimide, Schiff base) on bioink properties and cell interactions.
- To investigate synergistic effects of peptide mixtures on cell spreading.
Main Methods:
- Chemical modification of ALG and ADA polymers with YIGSR, RRETEWA, and IKVAV peptides using carbodiimide and Schiff base reactions.
- Characterization of modified bioinks using FTIR and NMR spectroscopy.
- Assessment of hydrogel properties (stability, viscosity, stiffness) and NIH/3T3 cell spreading on modified bioinks.
- Production of hydrogel capsules and droplets via manual processes and DoD printing.
Main Results:
- Modified bioinks, especially ADA-based, showed decreased stability, viscosity, and stiffness.
- Carbodiimide coupling was effective for ADA but not ALG; Schiff base coupling showed promise for both.
- Enhanced NIH/3T3 cell spreading was observed with carbodiimide-modified ADA, Schiff base-modified ADA, and PEG-Mal-modified ADA.
- A novel mixture of all peptides demonstrated synergistic effects, leading to higher cell aspect ratios compared to single-peptide modifications.
Conclusions:
- Peptide modification of ALG and ADA bioinks is crucial for improving cell-material interactions in 3D bioprinting.
- The selection and combination of peptides significantly influence bioink performance and cell behavior.
- Modified ADA bioinks, particularly with peptide mixtures, show substantial potential for advanced bioprinting applications.
