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Updated: Jun 27, 2025

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
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IGF2BP2 modulates autophagy and serves as a prognostic marker in glioma

Ning Li1,2, Limei Deng1,3, Yuming Zhang2

  • 1Department of Hematology Affiliated Hospital of Guangdong Medical University Zhanjiang Guangdong China.

Ibrain
|April 29, 2024
PubMed

Insights

Insulin-like growth factor 2 messenger RNA-binding protein 2 (IGF2BP2) promotes glioma growth and resistance to chemotherapy. Reducing IGF2BP2 levels inhibits tumor cell growth and enhances sensitivity to temozolomide, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma, a malignant brain tumor, lacks effective therapeutic targets due to poorly understood development mechanisms.
  • Identifying novel molecular targets is crucial for improving glioma treatment outcomes.

Purpose of the Study:

  • To investigate the role of insulin-like growth factor 2 messenger RNA-binding protein 2 (IGF2BP2) in glioma progression.
  • To explore IGF2BP2 as a potential therapeutic target for glioma treatment.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) database for IGF2BP2 expression and patient survival in low-grade glioma (LGG).
  • In vitro studies involving IGF2BP2 knockdown to assess effects on cell viability, cell cycle, colony formation, and autophagy (mCherry-GFP-LC3 assay, Western blot).
  • Immunohistochemistry to correlate IGF2BP2 and p62 expression in patient samples; drug sensitivity assays (Cell Counting Kit-8) with temozolomide.

Main Results:

  • Elevated IGF2BP2 expression correlated with shorter survival in LGG patients.
  • IGF2BP2 knockdown impaired cell viability, induced G0/G1 arrest, reduced colony formation, and increased autophagy.
  • IGF2BP2 knockdown reduced p62 levels and increased the LC3-II/LC3-I ratio, indicating enhanced autophagy.
  • Positive correlation between IGF2BP2 and p62 expression observed in glioma samples.
  • IGF2BP2 expression linked to drug resistance markers; IGF2BP2 knockdown sensitized cells to temozolomide.

Conclusions:

  • IGF2BP2 plays a significant role in glioma progression by modulating autophagy.
  • IGF2BP2 is associated with chemoresistance in glioma.
  • Targeting IGF2BP2 presents a promising strategy for enhancing glioma chemosensitization and therapy.
Keywords:
IGF2BP2LC3‐І/ІІautophagygliomap62

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