High Throughput Bioprinting Using Decellularized Adipose Tissue-Based Hydrogels for 3D Breast Cancer Modeling

Area of Science:

• Biomaterials Science
• Biotechnology
• Cancer Research

Background:

• 3D bioprinting offers rapid fabrication for high throughput in vitro drug screening.
• Decellularized extracellular matrix (dECM) hydrogels mimic tumor microenvironments for cancer research.
• Decellularized adipose tissue (DAT) hydrogels present a promising biomaterial for 3D cancer modeling.

Purpose of the Study:

• To develop a high throughput bioprinting method for 3D breast cancer models using DAT hydrogels.
• To compare detergent-based and detergent-free decellularization protocols for caprine adipose tissue.
• To assess the efficacy of DAT hydrogel for 3D cancer modeling and in vitro drug screening.

Main Methods:

• Comparative analysis of decellularization protocols for adipose tissue.
• Characterization of DAT hydrogel properties (histological, biochemical, morphological, biological, rheological).
• Optimization of bioprinting parameters for 3D breast cancer model fabrication.
• In vitro drug screening using 5-fluorouracil on 3D bioprinted microtumors.

Main Results:

• DAT hydrogel demonstrated cytocompatibility and suitable rheological properties for bioprinting.
• Successful fabrication of 3D breast cancer models using high throughput bioprinting in a 96-well plate format.
• Demonstrated utility of 3D bioprinted microtumors for in vitro drug screening.

Conclusions:

• High throughput bioprinting of DAT hydrogel is effective for creating 3D breast cancer models.
• This method facilitates rapid in vitro drug screening and holds potential for personalized disease models.
• The developed platform could advance downstream clinical applications in drug development and personalized medicine.