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The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy

  • 0Department of Obstetrics and Gynecology, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou, China.
Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology +

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April 30, 2024

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April 30, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Intrahepatic cholestasis of pregnancy (ICP) in rats showed decreased inositol 1,4,5-trisphosphate receptor (InsP3R) expression and lower serum calcium (Ca2+) levels. Lower Ca2+ correlated with higher glycocholic acid, suggesting a role in ICP pathogenesis.

Area Of Science

  • Biochemistry
  • Obstetrics
  • Pathology

Background

  • Intrahepatic cholestasis of pregnancy (ICP) affects maternal and fetal health.
  • Understanding ICP mechanisms is crucial for developing effective treatments.
  • This study investigates key molecular and biochemical changes in an ICP rat model.

Purpose Of The Study

  • To evaluate serum calcium (Ca2+) levels in an ICP rat model.
  • To assess inositol 1,4,5-trisphosphate receptor (InsP3R) expression in liver tissue of ICP rats.
  • To explore the relationship between Ca2+ and bile acid levels in ICP.

Main Methods

  • An ICP rat model was established using hormonal injections.
  • Liver tissue and serum samples were collected from control and ICP groups.
  • Inositol 1,4,5-trisphosphate receptor (InsP3R) expression was analyzed using qPCR and immunohistochemistry.
  • Serum Ca2+, glycocholic acid, and bile acid levels were quantified via ELISA.

Main Results

  • Inositol 1,4,5-trisphosphate receptor (InsP3R) mRNA and protein levels were significantly reduced in ICP model rats.
  • Serum levels of glycocholic acid and total bile acids were significantly elevated in the ICP group.
  • Serum Ca2+ levels were significantly lower in the ICP group and negatively correlated with glycocholic acid levels.

Conclusions

  • ICP in rats is characterized by decreased InsP3R expression and reduced serum Ca2+.
  • The observed decrease in Ca2+ is linked to elevated bile acid levels, particularly glycocholic acid.
  • These findings offer insights into ICP pathophysiology and potential therapeutic targets.

Keywords:

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