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The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids
Xueping Sun1,2, Meenu Singla-Rastogi3, Jingwen Wang1,2
1Institute of Crop Germplasm Resources (Biotechnology Research Center), Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong 250100, PR China.
Nucleic Acids Research
|April 30, 2024
Summary
Chloroplast ribosome biogenesis involves the YqeH-type GTPase Brassinazole-Insensitive Pale Green2 (BPG2) and ribosomal protein uS10c, which interact within nucleoids to regulate ribosome assembly and function.
Area of Science:
- Plant Biology
- Molecular Biology
- Cell Biology
Background:
- Chloroplasts contain ribosomal proteins (RPs) and ribosome biogenesis factors (RBFs) in nucleoids, suggesting a link between nucleoids and ribosome production.
- Brassinazole-Insensitive Pale Green2 (BPG2), a YqeH-type GTPase, is identified as a chloroplast nucleoid-associated RBF in Arabidopsis.
Purpose of the Study:
- To investigate the relationship between chloroplast nucleoids and BPG2-mediated ribosome biogenesis.
- To elucidate how BPG2 targets ribosomes and its role in ribosome assembly.
Main Methods:
- Investigated the interaction between BPG2 and ribosomal protein uS10c in chloroplast nucleoids.
- Analyzed the impact of uS10c haploinsufficiency on plant development and chloroplasts.
- Examined the association of BPG2 and uS10c with different ribosomal particles (30S, polysomes).
- Assessed the effects of BPG2 and uS10c mutations on rRNA processing and protein accumulation.
Main Results:
- BPG2 interacts with the essential plastid ribosomal protein uS10c in chloroplast nucleoids independently of ribosomal RNA (rRNA).
- uS10c is a haploinsufficient gene, with heterozygous deletion causing variegated shoots and chlorophyll aggregation.
- BPG2 exclusively binds to 30S ribosomal particles, while uS10c integrates into 30S particles and is found in polysomes.
- The absence of uS10c disrupts BPG2's targeting to nucleoids, causing it to diffuse within chloroplasts.
- Loss of BPG2 function or heterozygous uS10c deletion impairs rRNA processing, reduces plastid protein levels, and triggers chloroplast signaling.
Conclusions:
- The uS10c-BPG2 module plays a crucial role in mediating ribosome biogenesis within chloroplast nucleoids.
- This interaction is essential for proper ribosome assembly, rRNA processing, and maintaining chloroplast function.
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