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Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy in the Visible Range
Tetsu Tamura1, Phillip C McCann1, Ryo Nishiyama1
1Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan.
The Journal of Physical Chemistry Letters
|April 30, 2024
Summary
Visible FLETCHERS (vFLETCHERS) expands fluorescence-encoded vibrational spectroscopy to visible light, enabling sensitive analysis of more molecules. This technique enhances versatility for diverse applications, including biological imaging.
Area of Science:
- Chemical Physics
- Spectroscopy
- Biophotonics
Background:
- Fluorescence-encoded vibrational spectroscopy offers high sensitivity and chemical specificity.
- Previous fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS) was limited to near-infrared excitation, restricting its applicability.
- A scarcity of suitable fluorophores in the near-infrared range limited broader investigations of FLETCHERS.
Purpose of the Study:
- To extend the capabilities of FLETCHERS into the visible light spectrum.
- To enhance the versatility of fluorescence-encoded vibrational spectroscopy for a wider range of molecules.
- To demonstrate the potential of the new method for sensitive biological imaging.
Main Methods:
- Development and application of visible FLETCHERS (vFLETCHERS) using a noncollinear optical parametric amplifier.
- Excitation of fluorophores in the visible range (600-700 nm).
- Acquisition of individual Raman spectra from multiple visible fluorophores.
Main Results:
- Successfully extended FLETCHERS into the visible range (vFLETCHERS).
- Acquired distinct Raman spectra from five different visible fluorophores.
- Demonstrated the enhanced versatility of the technique for various molecules.
Conclusions:
- vFLETCHERS significantly broadens the applicability of fluorescence-encoded vibrational spectroscopy.
- The method shows promise for highly sensitive supermultiplexed imaging in biological research.
- This advancement opens new avenues for molecular analysis and imaging in diverse scientific fields.
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