Major Facilitator Superfamily Domain Containing 12 Is Overexpressed in Lung Cancer and Exhibits an Oncogenic Role in Lung Adenocarcinoma Cells
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View abstract on PubMed
Summary
This summary is machine-generated.Major facilitator superfamily domain containing 12 (MFSD12) is elevated in lung adenocarcinoma (LUAD), promoting cancer progression and poor survival. Inhibiting MFSD12 reduces LUAD cell proliferation, migration, and stemness, identifying it as a potential therapeutic target.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Genomics
Background
- Major facilitator superfamily domain containing 12 (MFSD12) is known to regulate lysosomal cysteine import and support melanoma cell growth.
- The role of MFSD12 in lung cancer, specifically lung adenocarcinoma (LUAD), is not well understood.
Purpose Of The Study
- To investigate the expression patterns of MFSD12 in various cancers, with a focus on LUAD.
- To determine the correlation between MFSD12 expression and clinicopathological features, patient survival, and immune infiltration in LUAD.
- To elucidate the functional role of MFSD12 in LUAD cell proliferation, migration, invasion, and stemness.
Main Methods
- Utilized TIMER and UALCAN databases for analyzing MFSD12 expression across cancers and LUAD patient data.
- Employed R package survival and CIBERSORT for survival analysis and immune cell infiltration assessment in LUAD.
- Performed in vitro experiments including CCK-8, colony formation, cell cycle, apoptosis, Transwell, Western blotting, and tumor sphere assays after MFSD12 knockdown in PC9 LUAD cells.
Main Results
- MFSD12 mRNA levels were significantly upregulated in multiple cancer types, including LUAD.
- In LUAD, MFSD12 expression correlated positively with advanced cancer stage, nodal metastasis, and infiltration of immune cells.
- High MFSD12 expression was associated with poorer patient survival.
- MFSD12 knockdown in PC9 cells led to reduced proliferation, colony formation, migration, invasion, and stemness, alongside cell cycle arrest and increased apoptosis.
Conclusions
- MFSD12 is overexpressed in LUAD and acts as an oncogene.
- MFSD12 promotes LUAD progression by enhancing cell proliferation, migration, invasion, and stemness.
- MFSD12 may serve as a prognostic biomarker and a potential therapeutic target for LUAD.
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