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Updated: Jun 27, 2025
An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
Published on: April 21, 2014
Long-term Intravital Investigation of an Orthotopic Glioma Mouse Model via Optical Coherence Tomography Angiography
Kaili Zheng1,2, Guangxing Wang1,2, Kangwei Zhou3
1State Key Laboratory of Vaccines for Infectious Diseases, Center for Molecular Imaging and Translational Medicine, Xiang An Biomedicine Laboratory, School of Public Health, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, P.R. China.
Optical coherence tomography angiography (OCTA) noninvasively visualizes brain tumor microvasculature. This study observed decreasing microvessels in a glioma mouse model, highlighting OCTA
Area of Science:
- Neuro-oncology
- Medical Imaging
- Vascular Biology
Background:
- Brain tumor microvasculature is crucial for cancer research and clinical practice.
- Existing clinical imaging methods lack noninvasive, high-precision monitoring of brain microvasculature without contrast agents.
- Understanding microvascular changes is key to tracking tumor development and treatment response.
Purpose of the Study:
- To investigate the characteristics of brain microvasculature during tumor development.
- To evaluate the utility of optical coherence tomography angiography (OCTA) for in vivo imaging.
- To establish an orthotopic glioma mouse model for studying microvascular dynamics.
Main Methods:
- Established an orthotopic glioma mouse model using U87-MG-luc cells.
- Employed optical coherence tomography angiography (OCTA) for microvascular characterization.
- Monitored microvascular progression over a 14-day period.
Main Results:
- Successfully generated and validated an orthotopic glioma mouse model via bioluminescence imaging and MRI.
- Observed a progressive decrease in microvessels within the tumor area over 14 days.
- Documented the microvascular changes occurring from the tumor center to the periphery.
Conclusions:
- Optical coherence tomography angiography (OCTA) is a promising tool for noninvasive in vivo visualization of brain microvasculature.
- OCTA provides high-precision imaging without the need for exogenous contrast agents.
- This technique can aid in studying brain tumor development and vascular dynamics.
