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Related Experiment Video

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Absolute quantification methods for Prostate-Specific antigen by Isotope-Dilution mass spectrometry.

Jianhui Wu1, Jianyi Liu1, Haofeng Sun2

  • 1Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China; School of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|May 1, 2024
PubMed
Summary

Standardizing prostate-specific antigen (PSA) detection is crucial for accurate prostate cancer diagnosis. This study developed traceable quantification methods for PSA, improving clinical test reliability.

Keywords:
Consistency assessmentLC–MS/MSProstate-specific antigen

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Prostate-specific antigen (PSA) is a key biomarker for prostate cancer, but its complex nature hinders standardized detection.
  • Variability in PSA detection results impacts clinical accuracy and reliability.

Purpose of the Study:

  • To develop and validate accurate, traceable quantification methods for prostate-specific antigen (PSA).
  • To establish a foundation for high-order reference materials to improve clinical PSA testing.

Main Methods:

  • Quantification of free PSA (fPSA) using amino acid analysis coupled with isotope-dilution mass spectrometry (AAA-IDMS).
  • Quantification using enzymatic hydrolysis combined with isotope-dilution mass spectrometry (IDMS).
  • Detection of amino acids and peptides via liquid chromatography-mass spectrometry with multiple reaction monitoring (LC-MS/MRM).

Main Results:

  • Achieved SI-traceable quantification of PSA mass concentration using AAA-IDMS and enzymatic hydrolysis-IDMS.
  • AAA-IDMS yielded (75.3 ± 1.5) µg/g PSA; enzymatic hydrolysis-IDMS yielded (74.7 ± 1.7) µg/g PSA.
  • Weighted average PSA mass concentration was (75.0 ± 1.6) µg/g, with validated consistency between methods.

Conclusions:

  • The developed methods provide absolute quantitative accuracy and traceability for PSA.
  • This work supports the creation of reference materials to enhance the accuracy, reliability, and consistency of clinical PSA tests.