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NETosis-Inspired Cell Surface-Constrained Framework Nucleic Acids Traps (FNATs) for Cascaded Extracellular
Hangsheng Gong1, Yihan Zhang1, Yuan Xue1
1School of Life Sciences, Anhui Medical University, Hefei, Anhui, 230032, China.
Angewandte Chemie (International Ed. in English)
|May 1, 2024
Summary
Researchers developed framework nucleic acid traps (FNATs) inspired by neutrophil extracellular traps (NETs). These DNA devices recognize extracellular signals like ATP, inhibit cell migration, and induce cell destruction, offering new tools for molecular medicine.
Area of Science:
- Biotechnology
- Molecular Biology
- Nanotechnology
Background:
- Neutrophils release neutrophil extracellular traps (NETs) to capture pathogens.
- NETosis is a key immune response involving DNA release.
- Existing methods lack precise control over cellular behavior and molecular recognition.
Purpose of the Study:
- To develop programmable, cell surface-constrained DNA nanostructures (FNATs).
- To enable dynamic recognition of extracellular signaling molecules.
- To modulate cellular behavior, including migration and self-destruction.
Main Methods:
- Design and synthesis of framework nucleic acid traps (FNATs).
- Programming FNATs for adenosine triphosphate (ATP) recognition.
- Integration of photosensitizer chlorin e6 (Ce6) for photodynamic therapy.
- In situ self-assembly of FNAs on target cell surfaces.
Main Results:
- FNATs demonstrated programmable recognition of extracellular ATP.
- In situ FNA assembly on cell surfaces inhibited target cell migration.
- Activation of Ce6 induced targeted cell self-destruction.
- The platform allows visualization of extracellular activities and manipulation of cellular behavior.
Conclusions:
- FNATs represent a novel DNA-based platform for dynamic molecular recognition and cellular behavior modulation.
- This technology offers potential applications in diagnostics and therapeutics.
- Programmable DNA nanodevices provide new tools for manipulating cellular functions in situ.
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