Ethanol responsive lnc171 promotes migration and invasion of HCC cells via mir-873-5p/ZEB1 axis
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View abstract on PubMed
Summary
This summary is machine-generated.Long noncoding RNA RP11-171K16.5 (lnc171) is upregulated in hepatocellular carcinoma (HCC) and promotes cancer cell migration and invasion. Lnc171 regulates ZEB1 expression via sponging mir-873-5p, and is responsive to ethanol.
Area Of Science
- Oncology
- Molecular Biology
- Gene Regulation
Background
- Long noncoding RNAs (lncRNAs) are critical regulators in human cancer development.
- lncRNAs serve as potential diagnostic and prognostic biomarkers for various cancers.
- The role of RP11-171K16.5 (lnc171) in hepatocellular carcinoma (HCC) requires further investigation.
Purpose Of The Study
- To confirm the expression and elucidate the molecular mechanism of lnc171 in HCC.
- To investigate the interaction between lnc171, mir-873-5p, and ZEB1 in HCC.
- To determine the effect of ethanol on lnc171 expression and its downstream targets in HCC.
Main Methods
- RNA sequencing for differential lncRNA expression analysis.
- Quantitative real-time PCR (qRT-PCR) for gene expression validation.
- Transwell and luciferase reporter assays to study cellular functions and binding interactions.
Main Results
- lnc171 was significantly upregulated in HCC tissues.
- Knockdown of lnc171 inhibited HCC cell migration and invasion.
- lnc171 sponges mir-873-5p, leading to upregulation of its target gene ZEB1, enhancing HCC cell migration and invasion.
- Ethanol stimulation increased lnc171 expression, impacting the ceRNA network in HCC cells.
Conclusions
- lnc171 is an ethanol-responsive factor implicated in HCC development.
- lnc171 plays a crucial role in HCC progression through its interaction with mir-873-5p.
- lnc171 may serve as a potential therapeutic target for HCC.
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