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Updated: Jun 27, 2025

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Tuning synaptic strength by regulation of AMPA glutamate receptor localization
Imogen Stockwell1, Jake F Watson2, Ingo H Greger1
1Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK.
None:
Long-term potentiation (LTP) of excitatory synapses is a leading model to explain the concept of information storage in the brain. Multiple mechanisms contribute to LTP, but central amongst them is an increased sensitivity of the postsynaptic membrane to neurotransmitter release. This sensitivity is predominantly determined by the abundance and localization of AMPA-type glutamate receptors (AMPARs). A combination of AMPAR structural data, super-resolution imaging of excitatory synapses, and an abundance of electrophysiological studies are providing an ever-clearer picture of how AMPARs are recruited and organized at synaptic junctions. Here, we review the latest insights into this process, and discuss how both cytoplasmic and extracellular receptor elements cooperate to tune the AMPAR response at the hippocampal CA1 synapse.
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