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Updated: Jul 22, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Multiple reports on the causal relationship between various chronic pain and gut microbiota: a two-sample Mendelian
Yuxin Cai1,2, Shuyang Wen1,2,3, Jinjing Hu1,2
1Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Frontiers in Neuroscience
|May 2, 2024
Summary
This study reveals a causal link between gut microbiota and chronic pain. Specific gut bacteria and metabolites influence pain risk, offering new insights into pain mechanisms.
Area of Science:
- Microbiome research
- Genetics
- Pain medicine
Background:
- The connection between gut microbiota and chronic pain is suggested but not fully understood.
- Investigating the causal relationship requires advanced analytical methods.
Purpose of the Study:
- To explore the potential causal relationship between gut microbiota and chronic pain at 12 specific locations.
- To identify specific microbial taxa and metabolites associated with pain risk.
Main Methods:
- Utilized UK Biobank and FinnGen GWAS data for pain information.
- Categorized gut microbiota at multiple taxonomic levels (phylum to genus).
- Performed Mendelian Randomization (MR) analysis to infer causality.
Main Results:
- Identified direct links between gut microbiota genetic susceptibility and pain at 12 sites.
- Serotonin (5-HT) and Glycine associated with higher extremity pain risk.
- Bifidobacteriaceae family linked to lower migraine risk; Oxalobacter genus linked to increased low back pain risk.
Conclusions:
- MR analysis confirmed a causal relationship between gut microbiota and pain.
- Findings highlight the microbiota's role in pain mechanisms and clinical implications.
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