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Updated: Jun 27, 2025

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Paclitaxel inhibits proliferation by negatively regulating Cdk1-cell cycle axis in rat airway smooth muscle cells

Bo Xiao1,2,3,4, Zhiheng Huang2,3, Liangxian Li5

  • 1Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Guilin Medical University, Guilin, China.

The Journal of Asthma : Official Journal of the Association for the Care of Asthma
|May 2, 2024
PubMed
Abstract

Insights

Paclitaxel inhibits airway smooth muscle cell proliferation by arresting the cell cycle at G2/M phase and down-regulating Cdk1. This study reveals paclitaxel

Area of Science:

  • Cell Biology
  • Pharmacology

Background:

  • Paclitaxel is known for its anti-proliferative and apoptosis-inducing effects.
  • The impact of paclitaxel on airway smooth muscle cells (ASMCs) remains unexplored.

Purpose of the Study:

  • To investigate the effects of paclitaxel on ASMC proliferation and apoptosis.
  • To elucidate the underlying molecular mechanisms, including cell cycle regulation and Cdk1 expression.

Main Methods:

  • Primary rat ASMCs were utilized.
  • Cell Counting Kit-8 and Edu assays assessed proliferation.
  • Flow cytometry analyzed cell cycle and apoptosis.
  • qRT-PCR, Western blotting, and immunostaining evaluated Cdk1 expression.

Main Results:

  • Paclitaxel inhibited ASMC proliferation in a dose- and time-dependent manner.
  • Cell cycle arrest at the G2/M phase was observed.
  • Paclitaxel treatment led to decreased Cdk1 expression.
  • No significant induction of ASMC apoptosis was detected.

Conclusions:

  • Paclitaxel inhibits ASMC proliferation.
  • This inhibition is partly mediated by the negative regulation of the Cdk1-cell cycle axis.
  • Paclitaxel does not induce apoptosis in ASMCs.
Keywords:
ASMCsCdk1Paclitaxelcell cyclecell proliferation

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