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Red-Light-Induced Genetic System for Control of Extracellular Electron Transfer.

Fengjie Zhao1, Christina M Niman1, Ghazaleh Ostovar1

  • 1Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States.

ACS Synthetic Biology
|May 2, 2024
PubMed
Summary
This summary is machine-generated.

Researchers adapted optogenetics for Shewanella oneidensis, enabling precise control over gene expression using red light. This advancement facilitates new synthetic biology applications in electrogenic bacteria.

Keywords:
Shewanella oneidensis MR-1cytochromeselectrochemical measurementsextracellular electron transferoptogeneticsred light

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Area of Science:

  • Synthetic Biology
  • Microbial Engineering
  • Optogenetics

Background:

  • Optogenetics offers precise spatiotemporal control of gene expression.
  • Existing light-inducible systems require adaptation for specific bacterial hosts.
  • Shewanella oneidensis is a key electrogenic bacterium with potential biotechnological applications.

Purpose of the Study:

  • To develop and adapt a red-light-inducible transcription factor for Shewanella oneidensis.
  • To enable differential gene expression in response to red light.
  • To expand synthetic biology tools for S. oneidensis.

Main Methods:

  • Adaptation of the iLight optogenetic system for S. oneidensis.
  • Utilized a thermodynamic model and promoter engineering for system optimization.
  • Engineered an inverted iLight circuit with a repressor for red-light-activated gene expression.

Main Results:

  • Successfully adapted and optimized a red-light-inducible system in S. oneidensis.
  • Demonstrated differential gene expression under red light and in darkness.
  • Showcased simultaneous use of red- and blue-light optogenetic circuits.
  • Applied the inverted iLight circuit to control extracellular electron transfer.

Conclusions:

  • The developed optogenetic tools enhance synthetic biology capabilities in S. oneidensis.
  • This work provides a foundation for advanced applications leveraging electrogenic bacteria.
  • Precise genetic control in S. oneidensis is now achievable with light-inducible systems.