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Single-cell analyzing of tumor microenvironment and cell adhesion between early and late-stage lung cancer

  • 0Department of Thoracic Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, China.
Molecular Immunology +

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May 2, 2024

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May 2, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Tensin 1 (TNS1) is identified as a key adhesive molecule in early-stage lung adenocarcinoma (LUAD). High TNS1 expression correlates with better survival and may serve as a novel prognostic marker for LUAD patients.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genomics

Background

  • Lung adenocarcinoma (LUAD) is a heterogeneous cancer with a high mortality rate.
  • The tumor microenvironment (TME) heterogeneity and factors driving LUAD progression remain incompletely understood.
  • Understanding early-stage LUAD progression is crucial for developing effective therapeutic strategies.

Purpose Of The Study

  • To identify key molecular factors controlling tumor migration in early-stage LUAD.
  • To investigate the role of cell-cell communication and gene expression in LUAD progression.
  • To evaluate Tensin 1 (TNS1) as a potential prognostic marker for LUAD.

Main Methods

  • Comprehensive analysis of single-cell RNA sequencing (scRNA-seq) data from LUAD patients.
  • Cell-cell communication analysis, cell cycle analysis, and Non-negative Matrix Factorization (NMF).
  • Survival analysis using TCGA database, immunofluorescence, immunohistochemistry, and pathway experiments.

Main Results

  • Significant expansion of epithelial cells and increased G2/M phase cells in late-stage LUAD.
  • Identification of Tensin 1 (TNS1) as an early-stage specific gene involved in cell adhesion.
  • High TNS1 expression correlated with better overall survival, co-expression with fibroblast/tumor markers, and activation of Akt/mTOR signaling.

Conclusions

  • TNS1 functions as an adhesive molecule in the early-stage LUAD immune microenvironment.
  • TNS1 expression levels are significantly higher in early-stage LUAD tissues.
  • TNS1 shows potential as a novel prognostic biomarker for lung adenocarcinoma.

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