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COPII cage assembly factor Sec13 integrates information flow regulating endomembrane function in response to human

Frédéric Anglès1, Vijay Gupta1, Chao Wang1

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Sec13 regulates protein transport via the COPII system, impacting gene expression and protein stability. This protein is key to managing cellular information flow and membrane architecture, especially in response to human genetic variations.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The precise coordination of information flow for genome transit via endomembrane pathways by the coat complex II (COPII) system, particularly in response to human variation, is not fully understood.
  • Sec13 is a known component of the COPII cage assembly, but its broader roles in cellular processes remain to be fully elucidated.

Purpose of the Study:

  • To investigate the interactome of Sec13 and its role in coordinating cellular processes.
  • To understand how Sec13 levels influence protein trafficking, degradation, and membrane architecture in the context of human variation.

Main Methods:

  • Proteomic analysis of Sec13 interactors.
  • Examination of Sec13's effect on the ubiquitination and degradation of cystic fibrosis transmembrane conductance regulator (CFTR) variants.
  • Assessment of Sec13's impact on protein stability and cellular membrane architecture.

Main Results:

  • Sec13 interacts with multiple protein complexes involved in chromatin organization, transcription, translation, trafficking, and degradation.
  • Reduced Sec13 levels decreased CFTR ubiquitination and degradation, increasing its stability, suggesting a critical role in differentiating export from degradation.
  • COPII cage assembly at the ER Golgi intermediate compartment (ERGIC) is crucial for regulating degradation linked to COPI exchange.

Conclusions:

  • Sec13 acts as a master regulator coordinating information flow from genome to proteome.
  • Sec13 facilitates spatial and functional aspects of membrane architecture, responding to human genetic variations.
  • The findings highlight Sec13's critical role in cellular homeostasis and its potential implications in diseases arising from human variation.